HuLPr16008

Uniprot ID

COM1_HUMAN

Uniprot Acc

Q99708;O75371;

Protein name

DNA endonuclease RBBP8

Comment

FUNCTION:Endonuclease that cooperates with the MRN complex in processing meiotic and mitotic double-strand breaks by ensuring both resection and intrachromosomal association of the broken ends. Promotes ATR and RPA recruitment to DSBs in S/G2 phase and facilitates the generation of ssDNA. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control.||SUBUNIT:Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Interacts with MRN complex subunits MRE11 and RAD50.||INTERACTION:P38398:BRCA1; NbExp=5; IntAct=EBI-1263531, EBI-349905; Q6UWZ7:FAM175A; NbExp=1; IntAct=EBI-1263531, EBI-1263451;||SUBCELLULAR LOCATION:Nucleus. Note=Associates with sites of DNA damage in S/G2 phase.||PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylated at Thr-847 by CDK1, which is essential for the re

Subcellular localization

Nucleus.

Gene name

retinoblastoma binding protein 8

Protein sequence

MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGLQV KVTKLKQERILDAQRLEEFFTKNQQLREQQKVLHETIKV LEDRLRAGLCDRCAVTEEHMRKKQQEFENIRQQNLKLIT ELMNERNTLQEENKKLSEQLQQKIENDQQHQAAELECEE DVIPDSPITAFSFSGVNRLRRKENPHVRYIEQTHTKLEH SVCANEMRKVSKSSTHPQHNPNENEILVADTYDQSQSPM AKAHGTSSYTPDKSSFNLATVVAETLGLGVQEESETQGP MSPLGDELYHCLEGNHKKQPFEESTRNTEDSLRFSDSTS KTPPQEELPTRVSSPVFGATSSIKSGLDLNTSLSPSLLQ PGKKKHLKTLPFSNTCISRLEKTRSKSEDSALFTHHSLG SEVNKIIIQSSNKQILINKNISESLGEQNRTEYGKDSNT DKHLEPLKSLGGRTSKRKKTEEESEHEVSCPQASFDKEN AFPFPMDNQFSMNGDCVMDKPLDLSDRFSAIQRQEKSQG SETSKNKFRQVTLYEALKTIPKGFSSSRKASDGNCTLPK DSPGEPCSQECIILQPLNKCSPDNKPSLQIKEENAVFKI PLRPRESLETENVLDDIKSAGSHEPIKIQTRSDHGGCEL ASVLQLNPCRTGKIKSLQNNQDVSFENIQWSIDPGADLS QYKMDVTVIDTKDGSQSKLGGETVDMDCTLVSETVLLKM KKQEQKGEKSSNEERKMNDSLEDMFDRTTHEEYESCLAD SFSQAADEEEELSTATKKLHTHGDKQDKVKQKAFVEPYF KGDERETSLQNFPHIEVVRKKEERRKLLGHTCKECEIYY ADMPAEEREKKLASCSRHRFRYIPPNTPENFWEVGFPST QTCMERGYIKEDLDPCPRPKRRQPYNAIFSPKGKEQKT

Database cross reference

RefSeq Protein accession:NP_002885
RefSeq Protein gi:4506441

HLPP validation

Yes/No

Yes

Project name

French Liver;Human Liver Organelles;

GO:0005634;C:nucleus;TAS:ProtInc.

GO-F

GO:0003684;F:damaged DNA binding;IDA:UniProtKB. GO:0005515;F:protein binding;IPI:UniProtKB. GO:0000014;F:single-stranded DNA specific endodeoxyribonuclease activity;IMP:UniProtKB.

GO-P

GO:0000075;P:cell cycle checkpoint;TAS:ProtInc. GO:0010792;P:DNA double-strand break processing involved in repair via single-strand annealing;IMP:UniProtKB. GO:0007126;P:meiosis;IEA:UniProtKB-KW. GO:0006357;P:regulation of transcription from RNA polyme

MOD type1

Position1

Residue1

Source name1

source ID1

Source method

HLPP validation1 (Yes/no)

Quality score

HuLPr16008

PHOSPHORYLATION

327

S

Phospho-ELM

3723

HTP

N

HuLPr16008

PHOSPHORYLATION

664

S

Phospho-ELM

1025

LTP

N

HuLPr16008

PHOSPHORYLATION

745

S

Phospho-ELM

1026

LTP

N

HuLPr16008

PHOSPHORYLATION

679

S

Phospho-ELM

21538

HTP

N

HuLPr16008

PHOSPHORYLATION

679

S

PhosphoSitePlus

482164

LTP

N

HuLPr16008

PHOSPHORYLATION

847

T

PhosphoSitePlus

7113805

LTP

N

HuLPr16008

PHOSPHORYLATION

664

S

PhosphoSitePlus

448391

LTP

HTP

N

HuLPr16008

PHOSPHORYLATION

745

S

PhosphoSitePlus

448392

LTP

N

HuLPr16008

PHOSPHORYLATION

326

S

PhosphoSitePlus

10941801

LTP

N

HuLPr16008

PHOSPHORYLATION

327

S

PhosphoSitePlus

452473

LTP

N

HuLPr16008

PHOSPHORYLATION

349

S

PhosphoSitePlus

14159747

LTP

N

HuLPr16008

PHOSPHORYLATION

233

S

PhosphoSitePlus

4766293

LTP

N

HuLPr16008

PHOSPHORYLATION

723

S

PhosphoSitePlus

4766312

LTP

N

ATM_pathway

BARD1_signaling_events

E2F_transcription_factor_network

Notch-mediated_HES/HEY_network

Notch_signaling_pathway