HuLPr04046

Uniprot ID

ALKB1_HUMAN

Uniprot Acc

Q13686;Q8TAU1;Q9ULA7;

Protein name

Alkylated DNA repair protein alkB homolog 1

Comment

FUNCTION:Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.||CATALYTIC ACTIVITY:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.||COFACTOR:Binds 1 Fe(2+) ion per subunit.||SUBUNIT:Monomer. Interacts with DNAJB6 (By similarity).||SUBCELLULAR LOCATION:Mitochondrion. Nucleus (By similarity). Note=Mainly localizes in euchromatin, largely excluded from heteroch

Subcellular localization

Mitochondrion.Nucleus(By similarity).

Gene name

alkB, alkylation repair homolog 1 (E. coli)

Protein sequence

MGKMAAAVGSVATLATEPGEDAFRKLFRFYRQSRPGTADL EGVIDFSAAHAARGKGPGAQKVIKSQLNVSSVSEQNAYR AGLQPVSKWQAYGLKGYPGFIFIPNPFLPGYQWHWVKQC LKLYSQKPNVCNLDKHMSKEETQDLWEQSKEFLRYKEAT KRRPRSLLEKLRWVTVGYHYNWDSKKYSADHYTPFPSDL GFLSEQVAAACGFEDFRAEAGILNYYRLDSTLGIHVDRS ELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGD IMIMSGFSRLLNHAVPRVLPNPEGEGLPHCLEAPLPAVL PRDSMVEPCSMEDWQVCASYLKTARVNMTVRQVLATDQN FPLEPIEDEKRDISTEGFCHLDDQNSEVKRARINPDS

Database cross reference

RefSeq Protein accession:NP_006011
RefSeq Protein gi:87298840

HLPP validation

Yes/No

Yes

Project name

Human Liver Organelles;

GO:0005739;C:mitochondrion;IDA:UniProtKB.

GO-F

GO:0003906;F:DNA-(apurinic or apyrimidinic site) lyase activity;IDA:UniProtKB. GO:0008198;F:ferrous iron binding;IDA:UniProtKB. GO:0070579;F:methylcytosine dioxygenase activity;IDA:UniProtKB. GO:0016702;F:oxidoreductase activity, acting on single donors

GO-P

GO:0006307;P:DNA dealkylation involved in DNA repair;IDA:UniProtKB. GO:0080111;P:DNA demethylation;IDA:UniProtKB. GO:0070989;P:oxidative demethylation;IDA:UniProtKB. GO:0042245;P:RNA repair;IDA:UniProtKB.

TCR