HuLPr24052

Uniprot ID

GLT12_HUMAN

Uniprot Acc

Q8IXK2;Q5TCF7;Q8NG54;Q96CT9;Q9H771;

Protein name

Polypeptide N-acetylgalactosaminyltransferase 12

Comment

FUNCTION:Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc- Muc5AC glycopeptide, but no detectable activity to mono-GalNAc- glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.||CATALYTIC ACTIVITY:UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide.||COFACTOR:Manganese (By similarity).||COFACTOR:Calcium (By similarity).||PATHWAY:Protein modification; protein glycosylation.||SUBCELLULAR LOCATION:Golgi apparatus membrane; Single-pass type II membrane protein (By similarity).||ALTERNATIVE PRODUCTS:Event=Alternative splicing; Named isoforms=2; Name=1; IsoI

Subcellular localization

Golgi apparatus membrane;Single-pass type II membrane protein(By similarity).

Gene name

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12)

Protein sequence

MWGRTARRRCPRELRRGREALLVLLALLALAGLGSVLRAQ RGAGAGAAEPGPPRTPRPGRREPVMPRPPVPANALGARG EAVRLQLQGEELRLQEESVRLHQINIYLSDRISLHRRLP ERWNPLCKEKKYDYDNLPRTSVIIAFYNEAWSTLLRTVY SVLETSPDILLEEVILVDDYSDREHLKERLANELSGLPK VRLIRANKREGLVRARLLGASAARGDVLTFLDCHCECHE GWLEPLLQRIHEEESAVVCPVIDVIDWNTFEYLGNSGEP QIGGFDWRLVFTWHTVPERERIRMQSPVDVIRSPTMAGG LFAVSKKYFEYLGSYDTGMEVWGGENLEFSFRIWQCGGV LETHPCSHVGHVFPKQAPYSRNKALANSVRAAEVWMDEF KELYYHRNPRARLEPFGDVTERKQLRDKLQCKDFKWFLE TVYPELHVPEDRPGFFGMLQNKGLTDYCFDYNPPDENQI VGHQVILYLCHGMGQNQFFEYTSQKEIRYNTHQPEGCIA VEAGMDTLIMHLCEETAPENQKFILQEDGSLFHEQSKKC VQAARKESSDSFVPLLRDCTNSDHQKWFFKERML

Database cross reference

RefSeq Protein accession:NP_078918
RefSeq Protein gi:112807221

HLPP validation

Yes/No

Yes

Project name

Chinese Liver;Human Liver Organelles;

GO:0000139;C:Golgi membrane;IEA:UniProtKB-SubCell. GO:0016021;C:integral to membrane;IEA:UniProtKB-KW.

GO-F

GO:0004653;F:polypeptide N-acetylgalactosaminyltransferase activity;IEA:EC. GO:0005529;F:sugar binding;IEA:UniProtKB-KW.