HuLPr25425

Uniprot ID

ISG15_HUMAN

Uniprot Acc

P05161;Q5SVA4;Q7Z2G2;Q96GF0;

Protein name

Ubiquitin-like protein ISG15

Comment

FUNCTION:Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral act

Subcellular localization

Cytoplasm.Secreted.

Gene name

ISG15 ubiquitin-like modifier

Protein sequence

MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHA FQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCD EPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQ DDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG GTEPGGRS

Database cross reference

RefSeq Protein accession:NP_005092
RefSeq Protein gi:4826774

HLPP validation

Yes/No

Yes

Project name

Chinese Liver;Human Liver Organelles;

GO:0005829;C:cytosol;EXP:Reactome. GO:0005615;C:extracellular space;NAS:UniProtKB.

GO-F

GO:0005515;F:protein binding;IPI:IntAct.

GO-P

GO:0007267;P:cell-cell signaling;NAS:UniProtKB. GO:0044419;P:interspecies interaction between organisms;IEA:UniProtKB-KW. GO:0032020;P:ISG15-protein conjugation;IDA:HGNC. GO:0032480;P:negative regulation of type I interferon production;TAS:Reactome. GO

FSH