HuLPr27740

Uniprot ID

NANP_HUMAN

Uniprot Acc

Q8TBE9;B3KP12;Q5JYN8;Q8TE97;Q9Y3N0;

Protein name

N-acylneuraminate-9-phosphatase

Comment

CATALYTIC ACTIVITY:N-acylneuraminate 9-phosphate + H(2)O = N- acylneuraminate + phosphate.||COFACTOR:Magnesium.||ENZYME REGULATION:Inhibited by vanadate and calcium.||BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=0.09 mM for N-acetylneuraminate 9-P; KM=19.2 mM for fructose 1,6-P2; KM=2.7 mM for 6-P-gluconate; KM=5.9 mM for N-acetylglucosamine 6-P; Vmax=112 umol/min/mg enzyme with N-acetylneuraminate 9-P as substrate; Vmax=6.10 umol/min/mg enzyme with fructose 1,6-P2 as substrate; Vmax=2.79 umol/min/mg enzyme with 6-P-gluconate as substrate; Vmax=2.46 umol/min/mg enzyme with N-acetylglucosamine 6-P as substrate; Vmax=0.149 umol/min/mg enzyme with sorbitol 6-P as substrate; Vmax=0.140 umol/min/mg enzyme with 3-P glycerate as substrate; Vmax=0.095 umol/min/mg enzyme with P-serine as substrate; Vmax=0.094 umol/min/mg enzyme with glycerol 3-P as substrate; Vmax=0.090 umol/min/mg enzyme with ribulose 5-P as substrate; Vmax=0.072 umol/min/mg enzyme with N

Gene name

N-acetylneuraminic acid phosphatase

Protein sequence

MGLSRVRAVFFDLDNTLIDTAGASRRGMLEVIKLLQSKYH YKEEAEIICDKVQVKLSKECFHPYNTCITDLRTSHWEEA IQETKGGAANRKLAEECYFLWKSTRLQHMTLAEDVKAML TELRKEVRLLLLTNGDRQTQREKIEACACQSYFDAVVVG GEQREEKPAPSIFYYCCNLLGVQPGDCVMVGDTLETDIQ GGLNAGLKATVWINKNGIVPLKSSPVPHYMVSSVLELPA LLQSIDCKVSMST

Database cross reference

RefSeq Protein accession:NP_689880
RefSeq Protein gi:23308749

HLPP validation

Yes/No

Yes

Project name

Chinese Liver;

GO-F

GO:0050124;F:N-acylneuraminate-9-phosphatase activity;IDA:UniProtKB. GO:0008967;F:phosphoglycolate phosphatase activity;IEA:InterPro.

GO-P

GO:0046380;P:N-acetylneuraminate biosynthetic process;IDA:UniProtKB.

MOD type1

Position1

Residue1

Source name1

source ID1

Source method

HLPP validation1 (Yes/no)

Quality score

HuLPr27740

PHOSPHORYLATION

57

S

PhosphoSitePlus

18667142

HTP

N

Amino_Sugar_Metabolism