HuLPr27850

Uniprot ID

NDST2_HUMAN

Uniprot Acc

P52849;Q2TB32;Q59H89;

Protein name

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2

Comment

FUNCTION:Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate.||CATALYTIC ACTIVITY:3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.||BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=4.7 uM for K5 polysaccharide; KM=0.76 uM for N-acetylated HS-II;||PATHWAY:Glycan metabolism; heparan sulfate biosynthesis.||PATHWAY:Glycan metabolism; heparin biosynthesis.||SUBUNIT:Monomer (By similarity).||SUBCELLULAR LOCATION:Golgi apparatus membrane; Single-pass type II membrane protein (By similarity).||ALTERNATIVE PRODUCTS:Event=Alternative splicing; Named isoforms

Subcellular localization

Golgi apparatus membrane;Single-pass type II membrane protein(By similarity).

Gene name

N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2

Protein sequence

MLQLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVST SPKAKEPLPLPLGDCSSGGAAGPGPARPPVPPRPPRPPE TARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELA PGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELL DRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLG LRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHS TYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDG IQRVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYI LVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFT FNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWS HMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAP HHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYR RGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSI RGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVR FLQCWTRLRLQTLPPVPLAQKYFELFPQERSPLWQNPCD DKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSL HPAVTSSFPSPSTFEEIQFFNSPNYHKGIDWYMDFFPVP SNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLT NPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQTPLA LRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELR TNPAASMESIQKFLGITPFLNYTRTLRFDDDKGFWCQGL EGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNLELS KLLSRLGQPVPSWLREELQHSSLG

Database cross reference

RefSeq Protein accession:NP_003626
RefSeq Protein gi:4505353

HLPP validation

Yes/No

Yes

Project name

Human Liver Organelles;

GO:0000139;C:Golgi membrane;IEA:UniProtKB-SubCell. GO:0016021;C:integral to membrane;TAS:ProtInc.

GO-F

GO:0015016;F:[heparan sulfate]-glucosamine N-sulfotransferase activity;IEA:EC. GO:0016787;F:hydrolase activity;IEA:UniProtKB-KW.

MOD type1

Position1

Residue1

Source name1

source ID1

Source method

HLPP validation1 (Yes/no)

Quality score

HuLPr27850

ACETYLATION

588

K

PhosphoSitePlus

14366715

LTP

N

HuLPr27850

ACETYLATION

591

K

PhosphoSitePlus

14366719

LTP

N

IL2