HuLPr29714

Uniprot ID

PHOP1_HUMAN

Uniprot Acc

Q8TCT1;Q17RU6;

Protein name

Phosphoethanolamine/phosphocholine phosphatase

Comment

FUNCTION:Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.||CATALYTIC ACTIVITY:O-phosphoethanolamine + H(2)O = ethanolamine + phosphate.||CATALYTIC ACTIVITY:Phosphocholine + H(2)O = choline + phosphate.||COFACTOR:Magnesium.||BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=3 uM for PEA; KM=11.4 uM for PCho; pH dependence: Optimum pH is 6.7;||ALTERNATIVE PRODUCTS:Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8TCT1-1; Sequence=Displayed; Name=2; Synonyms=PHOSPHO1-3a; IsoId=Q8TCT1-2; Sequence=VSP 040624;||TISSUE SPECIFICITY:Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.||SIMILARITY:Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

Gene name

phosphatase, orphan 1

Protein sequence

MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDE NSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYL GEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEV ILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGL LALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFE RLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEA QKAEPSSFRASVVPWETAADVRLHLQQVLKSC

Database cross reference

RefSeq Protein accession:NP_001137276
RefSeq Protein gi:219689097

HLPP validation

Yes/No

Yes

Project name

Human Liver Organelles;

GO-F

GO:0046872;F:metal ion binding;IEA:UniProtKB-KW. GO:0033884;F:phosphoethanolamine/phosphocholine phosphatase activity;IEA:EC.

GO-P

GO:0030500;P:regulation of bone mineralization;IEA:UniProtKB-KW.

Phospholipid_Biosynthesis