HuLPr45090

Uniprot ID

S28A3_HUMAN

Uniprot Acc

Q9HAS3;A8K9Y4;B1AML0;

Protein name

Solute carrier family 28 member 3

Comment

FUNCTION:Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine.||BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=21.6 uM for uridine; KM=15.4 uM for cytidine; KM=21.2 uM for thymidine; KM=15.1 uM for adenosine; KM=43 uM for guanosine; KM=52.5 uM for inosine; Vmax=25.8 pmol/min/mg enzyme for uridine uptake; Vmax=32.8 pmol/min/mg enzyme for cytidine uptake; Vmax=24.2 pmol/min/mg enzyme for thymidine uptake; Vmax=30.4 pmol/min/mg enzyme for adenosine uptake; Vmax=51.4 pmol/min/mg enzyme for guanosine uptake; Vmax=44.8 pmol/min/mg enzyme for inosine uptake;||SUBCELLULAR LOCATION:Membrane; Multi-pass membrane protein (Pote

Subcellular localization

Membrane;Multi-pass membrane protein(Potential).

Gene name

solute carrier family 28 (sodium-coupled nucleoside transporter), member 3

Protein sequence

MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSR AVQSREHTNTKQDEEQVTVEQDSPRNREHMEDDDEEMQQ KGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVI SACVLNFHRALPLFVITVAAIFFVVWDHLMAKYEHRIDE MLSPGRRLLNSHWFWLKWVIWSSLVLAVIFWLAFDTAKL GQQQLVSFGGLIMYIVLLFLFSKYPTRVYWRPVLWGIGL QFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFV FGEKYKDHFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIR KVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRPYL PYITKSELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLT ASVMSAPASLAAAKLFWPETEKPKITLKNAMKMESGDSG NLLEAATQGASSSISLVANIAVNLIAFLALLSFMNSALS WFGNMFDYPQLSFELICSYIFMPFSFMMGVEWQDSFMVA RLIGYKTFFNEFVAYEHLSKWIHLRKEGGPKFVNGVQQY ISIRSEIIATYALCGFANIGSLGIVIGGLTSMAPSRKRD IASGAVRALIAGTVACFMTACIAGILSSTPVDINCHHVL ENAFNSTFPGNTTKVIACCQSLLSSTVAKGPGEVIPGGN HSLYSLKGCCTLLNPSTFNCNGISNTF

Database cross reference

RefSeq Protein accession:NP_001186562
RefSeq Protein gi:313851011

HLPP validation

Yes/No

Yes

Project name

Human Liver Organelles;

GO:0016021;C:integral to membrane;IEA:UniProtKB-KW. GO:0005886;C:plasma membrane;EXP:Reactome.

GO-F

GO:0001882;F:nucleoside binding;IEA:InterPro.

GO-P

GO:0055086;P:nucleobase, nucleoside and nucleotide metabolic process;TAS:Reactome.