HuLPr48290

Uniprot ID

VP33B_HUMAN

Uniprot Acc

Q9H267;Q96K14;Q9NRP6;Q9NSF3;

Protein name

Vacuolar protein sorting-associated protein 33B

Comment

FUNCTION:May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages.||SUBUNIT:Interacts with RAB11A and VIPAR. Interacts with M.tuberculosis PtpA.||INTERACTION:Q9H9C1:VIPAR; NbExp=4; IntAct=EBI-749072, EBI-749080;||SUBCELLULAR LOCATION:Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes. Colocalizes with M.tuberculosis PtpA in the cytosol of tuberculosis-infected macrophages and associates with phagosomes.||TISSUE SPECIFICITY:Ubiquitous; highly expressed in testis and low expression in the lung.||PTM:Phosphorylated on tyrosine residues. Dephosphorylation by M.tuberculosis PtpA is necessary to induce the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages.

Subcellular localization

Late endosome membrane;Peripheral membrane protein;Cytoplasmic side.Lysosome membrane;Peripheral membrane protein;Cytoplasmic side.

Gene name

vacuolar protein sorting 33 homolog B (yeast)

Protein sequence

MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLF IEADLMSPLDRIANVSILKQHEVDKLYKVENKPALSSNE QLCFLVRPRIKNMRYIASLVNADKLAGRTRKYKVIFSPQ KFYACEMVLEEEGIYGDVSCDEWAFSLLPLDVDLLSMEL PEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGI GRCAKMAYELWRNLEEEEDGETKGRRPEIGHIFLLDRDV DFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKS LKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYD RRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIM KKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQV SPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEH LLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAG KITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRD MAYVFGGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCS DFAFTDMTKEDKASSESLRLILVVFLGGCTFSEISALRF LGREKGYRFIFLTTAVTNSARLMEAMSEVKA

Database cross reference

RefSeq Protein accession:NP_061138
RefSeq Protein gi:119829191

HLPP validation

Yes/No

Yes

Project name

Chinese Liver;

GO:0031902;C:late endosome membrane;IEA:UniProtKB-SubCell. GO:0005765;C:lysosomal membrane;IEA:UniProtKB-SubCell. GO:0048471;C:perinuclear region of cytoplasm;IDA:UniProtKB. GO:0031091;C:platelet alpha granule;IDA:UniProtKB.

GO-F

GO:0005515;F:protein binding;IPI:UniProtKB.

GO-P

GO:0006944;P:cellular membrane fusion;IMP:UniProtKB. GO:0032418;P:lysosome localization;IDA:UniProtKB. GO:0032400;P:melanosome localization;IDA:UniProtKB. GO:0070889;P:platelet alpha granule organization;IMP:UniProtKB. GO:0015031;P:protein transport;IM