HuLPr48544

Uniprot ID

XRCC5_HUMAN

Uniprot Acc

P13010;A8K3X5;Q0Z7V0;Q4VBQ5;Q53HH7;Q7M4N0;Q9UCQ0;Q9UCQ1;

Protein name

X-ray repair cross-complementing protein 5

Comment

FUNCTION:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing th

Subcellular localization

Nucleus.Chromosome.

Gene name

X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)

Related liver disease name

Hepatocellular carcinoma

Protein sequence

MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITM FVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITV HRHLMLPDFDLLEDIESKIQPGSQQADFLDALIVSMDVI QHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKC DISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKG ITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLC VFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKK TWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQG FRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQ RRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVA IVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDL RQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEK TDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPI QQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQ VTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSV TSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLD TNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQE KVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKK FLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Database cross reference

RefSeq Protein accession:NP_066964
RefSeq Protein gi:10863945

HCC significant Proteins

Yes/No

Yes

Quality score

HLPP validation

Yes/No

Yes

Project name

Chinese Liver;French Liver;Human Fetal Liver;Human Liver Organelles;

GO:0043564;C:Ku70:Ku80 complex;IDA:UniProtKB. GO:0070419;C:nonhomologous end joining complex;IDA:UniProtKB. GO:0000783;C:nuclear telomere cap complex;TAS:BHF-UCL. GO:0005654;C:nucleoplasm;EXP:Reactome.

GO-F

GO:0005524;F:ATP binding;IEA:UniProtKB-KW. GO:0004003;F:ATP-dependent DNA helicase activity;TAS:ProtInc. GO:0003690;F:double-stranded DNA binding;TAS:ProtInc. GO:0010843;F:promoter binding;IDA:BHF-UCL. GO:0008022;F:protein C-terminus binding;IPI:UniPro

GO-P

GO:0006303;P:double-strand break repair via nonhomologous end joining;IMP:UniProtKB. GO:0019059;P:initiation of viral infection;EXP:Reactome. GO:0045892;P:negative regulation of transcription, DNA-dependent;IMP:UniProtKB. GO:0019047;P:provirus integrati

MOD type1

Position1

Residue1

Source name1

source ID1

Source method

HLPP validation1 (Yes/no)

Quality score

HuLPr48544

PHOSPHORYLATION

577

S

Phospho-ELM

8239

LTP

N

HuLPr48544

PHOSPHORYLATION

580

S

Phospho-ELM

8240

LTP

N

HuLPr48544

PHOSPHORYLATION

715

T

Phospho-ELM

8241

LTP

N

HuLPr48544

PHOSPHORYLATION

577

S

PhosphoSitePlus

452071

HTP

N

HuLPr48544

PHOSPHORYLATION

580

S

PhosphoSitePlus

452072

HTP

N

HuLPr48544

PHOSPHORYLATION

715

T

PhosphoSitePlus

452073

LTP

N

HuLPr48544

ACETYLATION

665

K

PhosphoSitePlus

11315881

HTP

N

HuLPr48544

ACETYLATION

660

K

PhosphoSitePlus

11315884

HTP

N

HuLPr48544

ACETYLATION

144

K

PhosphoSitePlus

11315887

LTP

N

HuLPr48544

ACETYLATION

195

K

PhosphoSitePlus

11315890

HTP

N

HuLPr48544

ACETYLATION

532

K

PhosphoSitePlus

11315893

HTP

N

HuLPr48544

ACETYLATION

338

K

PhosphoSitePlus

466166

LTP

N

HuLPr48544

PHOSPHORYLATION

255

S

PhosphoSitePlus

14160396

LTP

HTP

N

HuLPr48544

PHOSPHORYLATION

258

S

PhosphoSitePlus

14160399

LTP

HTP

N

HuLPr48544

MONO-METHYLATION

702

K

PhosphoSitePlus

2586333

LTP

N

HuLPr48544

ACETYLATION

265

K

PhosphoSitePlus

461638

LTP

N

HuLPr48544

ACETYLATION

332

K

PhosphoSitePlus

3856966

LTP

N

HuLPr48544

SUMOYLATION

568

K

PhosphoSitePlus

3278904

LTP

N

Coregulation_of_Androgen_receptor_activity

BARD1_signaling_events

DNA-PK_pathway_in_nonhomologous_end_joining

Regulation_of_Telomerase