HuLPr50229

Uniprot ID

NOS2_HUMAN

Uniprot Acc

P35228;A1L3U5;B7ZLY2;O60757;O94994;Q16263;Q16692;Q4TTS5;Q9UD42;

Protein name

Nitric oxide synthase, inducible

Comment

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.||CATALYTIC ACTIVITY: L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).||COFACTOR: Heme group (By similarity).||COFACTOR: Binds 1 FAD (By similarity).||COFACTOR: Binds 1 FMN (By similarity).||COFACTOR: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).||ENZYME REGULATION: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity (By similarity).||SUBUNIT: Homodimer. Binds SLC9A3R1.||ALTERNATIVE PRODUCTS: Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P35228-1; Sequenc

Gene name

nitric oxide synthase 2, inducible

Protein sequence

MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVT QDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPL SSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGS IMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEA KIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRN APRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYST NNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPD GSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANG RDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAV ANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNI LEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQN VTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMS GSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRP KRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATET GKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLL LVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFG LGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSG QEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVT WDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQN LQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQP ALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLP PCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRL EALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLS QLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQG PLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHP CILIGPGTGIAPFRSFWQQRLHDS

Database cross reference

RefSeq Protein accession:NP_000616
RefSeq Protein gi:24041029

GO:0005829; C:cytosol; IMP:BHF-UCL. GO:0005634; C:nucleus; ISS:BHF-UCL.

GO-F

GO:0034618; F:arginine binding; ISS:BHF-UCL. GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW. GO:0050660; F:flavin adenine dinucleotide binding; ISS:BHF-UCL. GO:0010181; F:FMN binding; ISS:BHF-UCL. GO:0020037; F:heme binding; ISS:BHF-UCL. GO:005066

GO-P

GO:0006527; P:arginine catabolic process; IDA:BHF-UCL. GO:0050829; P:defense response to Gram-negative bacterium; NAS:BHF-UCL. GO:0002227; P:innate immune response in mucosa; NAS:BHF-UCL. GO:0006809; P:nitric oxide biosynthetic process; IDA:BHF-UCL. GO

MOD type1

Position1

Residue1

Source name1

source ID1

Source method

HLPP validation1 (Yes/no)

Quality score

HuLPr50229

PHOSPHORYLATION

151

Y

PhosphoSitePlus

457593

LTP

N

HuLPr50229

PHOSPHORYLATION

745

S

PhosphoSitePlus

763800

LTP

HTP

N

HuLPr50229

PHOSPHORYLATION

868

Y

PhosphoSitePlus

3973918

LTP

N

HuLPr50229

PHOSPHORYLATION

1055

Y

PhosphoSitePlus

4115776

LTP

N